Assessment of tea plant (Camellia sinensis L.) accessions for pollen sources in natural crossing by using microsatellites

Tea (Camellia sinensis L. [O.] Kuntze) is a highly cross-pollinated and self-incompatible plant. Seeds can be harvested from specific individual mother plants in polyclonal tea gardens. Whether the pollen donor plays an important role seed formation remains unclear. This study aimed to identify male parents of 72 natural hybridized progenies (F1) one female parent on basis putative allele by using simple-sequence repeat (SSR) markers exclusion-likelihood method with Cervus 3.0 software. The genetic material, which comprised seven accessions C. L., was acquired Assamica planted Kayulandak garden Pagilaran plantation Batang District, Central Java, Indonesia, studied during 2019 2020. genotype PGL-15 used as parent, whereas six candidate genotypes PGL-10, GMB-9, GMB-7, TPS-93, GMB-11, TRI-2025 were parents. In this study, 13 SSR loci F1 obtained through hybridization between accessions. Results indicated that method, correctly predicted 100% parents, more effective than approach, only 34.72% plants.